The Structure of Glycinamide Ribotide” by Richard

Materials-The pigeon liver extract preparation and the substrates required for the synthesis of glycinamide ribotide have been described previously (l-4). Intestinal phosphatase, purified grade, was obtained from Pentex, Inc., Kankakee, Illinois. Adenosine-5’-phosphate was obtained from the Ernst Bischoff Company, Inc., Ivoryton, Connecticut. Adenosine-3’-phosphate was obtained from the Schwarz Laboratories, Inc., New York. Methods-For paper chromatography Whatman No. 1 paper was washed with 2 N acetic acid and then with 1O-4 M ethylenediamine sodium tetraacetate. The following solvent systems were employed for paper chromatography (all ratios are in volume proportions) : (1) n-butanol-17.6 N acetic acid-water (2: 1: I), (2) isopropanol-water (70:30) in an atmosphere of ammonia (0.35 ml. of concentrated ammonium hydroxide per liter of jar volume), (3) propanol-water (70: 30), (4) 1.0 M ammonium acetate, pH 7.5-95 per cent ethanol (30:75), (5) 95 per cent ethanol-O.025 M potassium borate, pH 8.2 (60:40), (6) 100 gm. of phenol plus 39.5 gm. of water, (7) tertiary amyl alcohol saturated with 0.05 M potassium phthalate, pH 6.0, and (8) 1.0 M ammonium acetate, pH 7.5-95 per cent ethanol (30: 60). In